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1. A microbiology laboratory isolates a Gram-negative rod from a wound infection. The organism is oxidase- negative, ferments lactose on MacConkey agar, and produces indole. The MOST likely identification is:
A) Proteus mirabilis
B) Serratia marcescens
C) Klebsiella pneumoniae
D) Escherichia coli
2. A patient develops a severe diarrheal illness after consuming raw seafood. Stool culture yields a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing blue-green colonies. The MOST significant virulence factor contributing to the severe, cholera-like diarrhea caused by this organism is:
A) Cholera toxin (CT)-like enterotoxin
B) Type III secretion system (T3SS)
C) Heat-labile toxin (LT)
D) Heat-stable enterotoxin (ST)
3. An outbreak of gastroenteritis occurs among attendees of a company picnic. Epidemiological investigation points towards contaminated potato salad. Stool cultures from affected individuals grow non-lactose fermenting, Gram-negative rods that are H2S positive on TSI agar. Which pathogen is most likely responsible?
A) Salmonella Enteritidis
B) Vibrio cholerae
C) Enterotoxigenic E. coli (ETEC)
D) Shigella sonnei
4. An immunocompromised patient develops a disseminated fungal infection. Tissue biopsy reveals small (2-4
µm) yeast cells within macrophages. The patient has a history of living in the Ohio and Mississippi River valleys. The MOST likely causative agent is:
A) Paracoccidioides brasiliensis
B) Blastomyces dermatitidis
C) Penicillium marneffei
D) Histoplasma capsulatum
5. Microscopic examination of a potassium hydroxide (KOH) preparation of skin scrapings from a patient with tinea versicolor typically reveals:
A) Septate hyphae and arthroconidia
B) Short, unbranched hyphae and globose yeast cells in clusters ("spaghetti and meatballs")
C) Broad, aseptate hyphae with sporangiophores
D) Cigar-shaped yeast cells
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: A | Question # 3 Answer: A | Question # 4 Answer: D | Question # 5 Answer: B |
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